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cdc25 ( twine ) mrna  (Cellscript Inc)

 
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    Cellscript Inc cdc25 ( twine ) mrna
    Interphase duration limits the establishment of heterochromatin. ( A ) Selected stills from live imaging of Halo-egg (shown in green) embryos injected with Cy5-labeled Histone proteins (shown in magenta). Egg dissociates from the chromatin upon mitotic chromosome condensation (150 sec). Bar, 3 µm. See Supplemental Movie S6 . ( B ) Arresting the cell cycle in interphase 13 permits continued accumulation of Egg and HP1a at heterochromatin. Embryos were filmed by confocal microscopy after injection of buffer or dsRNA against cyclins A, B, and B3. Bar, 2 µm. The beginning of cycle 13 was set as 0:00. See Supplemental Movie S7 . ( C–E ) Shortening interphase duration reduces HP1a accumulation. ( C ) Stills from confocal microscopy tracking GFP-HP1a in embryos laid from mothers either heterozygous or homozygous for grp. Note that grp embryos enter mitosis 13 before completing S phase, leading to anaphase bridges (17:00). Bar, 4 µm. The start of interphase 13 was set as 0:00. ( D ) Plot of the average number of HP1a foci per nucleus over time in control embryos (orange) or grp embryos (black). Each line represents data from a different embryo, and foci of GFP-HP1a were counted in ∼15 nuclei per embryo. ( E ) Inducing an early mitosis 14 by expression of twine <t>(cdc25)</t> interrupts heterochromatin formation. Embryos expressing GFP-HP1a were filmed following injection of twine mRNA during cycle 13. Bar, 3 µm. The beginning of cycle 14 was set as 0:00.
    Cdc25 ( Twine ) Mrna, supplied by Cellscript Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdc25 ( twine ) mrna/product/Cellscript Inc
    Average 90 stars, based on 1 article reviews
    cdc25 ( twine ) mrna - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Rapid embryonic cell cycles defer the establishment of heterochromatin by Eggless/SetDB1 in Drosophila"

    Article Title: Rapid embryonic cell cycles defer the establishment of heterochromatin by Eggless/SetDB1 in Drosophila

    Journal: Genes & Development

    doi: 10.1101/gad.321646.118

    Interphase duration limits the establishment of heterochromatin. ( A ) Selected stills from live imaging of Halo-egg (shown in green) embryos injected with Cy5-labeled Histone proteins (shown in magenta). Egg dissociates from the chromatin upon mitotic chromosome condensation (150 sec). Bar, 3 µm. See Supplemental Movie S6 . ( B ) Arresting the cell cycle in interphase 13 permits continued accumulation of Egg and HP1a at heterochromatin. Embryos were filmed by confocal microscopy after injection of buffer or dsRNA against cyclins A, B, and B3. Bar, 2 µm. The beginning of cycle 13 was set as 0:00. See Supplemental Movie S7 . ( C–E ) Shortening interphase duration reduces HP1a accumulation. ( C ) Stills from confocal microscopy tracking GFP-HP1a in embryos laid from mothers either heterozygous or homozygous for grp. Note that grp embryos enter mitosis 13 before completing S phase, leading to anaphase bridges (17:00). Bar, 4 µm. The start of interphase 13 was set as 0:00. ( D ) Plot of the average number of HP1a foci per nucleus over time in control embryos (orange) or grp embryos (black). Each line represents data from a different embryo, and foci of GFP-HP1a were counted in ∼15 nuclei per embryo. ( E ) Inducing an early mitosis 14 by expression of twine (cdc25) interrupts heterochromatin formation. Embryos expressing GFP-HP1a were filmed following injection of twine mRNA during cycle 13. Bar, 3 µm. The beginning of cycle 14 was set as 0:00.
    Figure Legend Snippet: Interphase duration limits the establishment of heterochromatin. ( A ) Selected stills from live imaging of Halo-egg (shown in green) embryos injected with Cy5-labeled Histone proteins (shown in magenta). Egg dissociates from the chromatin upon mitotic chromosome condensation (150 sec). Bar, 3 µm. See Supplemental Movie S6 . ( B ) Arresting the cell cycle in interphase 13 permits continued accumulation of Egg and HP1a at heterochromatin. Embryos were filmed by confocal microscopy after injection of buffer or dsRNA against cyclins A, B, and B3. Bar, 2 µm. The beginning of cycle 13 was set as 0:00. See Supplemental Movie S7 . ( C–E ) Shortening interphase duration reduces HP1a accumulation. ( C ) Stills from confocal microscopy tracking GFP-HP1a in embryos laid from mothers either heterozygous or homozygous for grp. Note that grp embryos enter mitosis 13 before completing S phase, leading to anaphase bridges (17:00). Bar, 4 µm. The start of interphase 13 was set as 0:00. ( D ) Plot of the average number of HP1a foci per nucleus over time in control embryos (orange) or grp embryos (black). Each line represents data from a different embryo, and foci of GFP-HP1a were counted in ∼15 nuclei per embryo. ( E ) Inducing an early mitosis 14 by expression of twine (cdc25) interrupts heterochromatin formation. Embryos expressing GFP-HP1a were filmed following injection of twine mRNA during cycle 13. Bar, 3 µm. The beginning of cycle 14 was set as 0:00.

    Techniques Used: Imaging, Injection, Labeling, Confocal Microscopy, Control, Expressing



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    cdc25 ( twine ) mrna  (Cellscript Inc)
    90
    Cellscript Inc cdc25 ( twine ) mrna
    Interphase duration limits the establishment of heterochromatin. ( A ) Selected stills from live imaging of Halo-egg (shown in green) embryos injected with Cy5-labeled Histone proteins (shown in magenta). Egg dissociates from the chromatin upon mitotic chromosome condensation (150 sec). Bar, 3 µm. See Supplemental Movie S6 . ( B ) Arresting the cell cycle in interphase 13 permits continued accumulation of Egg and HP1a at heterochromatin. Embryos were filmed by confocal microscopy after injection of buffer or dsRNA against cyclins A, B, and B3. Bar, 2 µm. The beginning of cycle 13 was set as 0:00. See Supplemental Movie S7 . ( C–E ) Shortening interphase duration reduces HP1a accumulation. ( C ) Stills from confocal microscopy tracking GFP-HP1a in embryos laid from mothers either heterozygous or homozygous for grp. Note that grp embryos enter mitosis 13 before completing S phase, leading to anaphase bridges (17:00). Bar, 4 µm. The start of interphase 13 was set as 0:00. ( D ) Plot of the average number of HP1a foci per nucleus over time in control embryos (orange) or grp embryos (black). Each line represents data from a different embryo, and foci of GFP-HP1a were counted in ∼15 nuclei per embryo. ( E ) Inducing an early mitosis 14 by expression of twine <t>(cdc25)</t> interrupts heterochromatin formation. Embryos expressing GFP-HP1a were filmed following injection of twine mRNA during cycle 13. Bar, 3 µm. The beginning of cycle 14 was set as 0:00.
    Cdc25 ( Twine ) Mrna, supplied by Cellscript Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdc25 ( twine ) mrna/product/Cellscript Inc
    Average 90 stars, based on 1 article reviews
    cdc25 ( twine ) mrna - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Interphase duration limits the establishment of heterochromatin. ( A ) Selected stills from live imaging of Halo-egg (shown in green) embryos injected with Cy5-labeled Histone proteins (shown in magenta). Egg dissociates from the chromatin upon mitotic chromosome condensation (150 sec). Bar, 3 µm. See Supplemental Movie S6 . ( B ) Arresting the cell cycle in interphase 13 permits continued accumulation of Egg and HP1a at heterochromatin. Embryos were filmed by confocal microscopy after injection of buffer or dsRNA against cyclins A, B, and B3. Bar, 2 µm. The beginning of cycle 13 was set as 0:00. See Supplemental Movie S7 . ( C–E ) Shortening interphase duration reduces HP1a accumulation. ( C ) Stills from confocal microscopy tracking GFP-HP1a in embryos laid from mothers either heterozygous or homozygous for grp. Note that grp embryos enter mitosis 13 before completing S phase, leading to anaphase bridges (17:00). Bar, 4 µm. The start of interphase 13 was set as 0:00. ( D ) Plot of the average number of HP1a foci per nucleus over time in control embryos (orange) or grp embryos (black). Each line represents data from a different embryo, and foci of GFP-HP1a were counted in ∼15 nuclei per embryo. ( E ) Inducing an early mitosis 14 by expression of twine (cdc25) interrupts heterochromatin formation. Embryos expressing GFP-HP1a were filmed following injection of twine mRNA during cycle 13. Bar, 3 µm. The beginning of cycle 14 was set as 0:00.

    Journal: Genes & Development

    Article Title: Rapid embryonic cell cycles defer the establishment of heterochromatin by Eggless/SetDB1 in Drosophila

    doi: 10.1101/gad.321646.118

    Figure Lengend Snippet: Interphase duration limits the establishment of heterochromatin. ( A ) Selected stills from live imaging of Halo-egg (shown in green) embryos injected with Cy5-labeled Histone proteins (shown in magenta). Egg dissociates from the chromatin upon mitotic chromosome condensation (150 sec). Bar, 3 µm. See Supplemental Movie S6 . ( B ) Arresting the cell cycle in interphase 13 permits continued accumulation of Egg and HP1a at heterochromatin. Embryos were filmed by confocal microscopy after injection of buffer or dsRNA against cyclins A, B, and B3. Bar, 2 µm. The beginning of cycle 13 was set as 0:00. See Supplemental Movie S7 . ( C–E ) Shortening interphase duration reduces HP1a accumulation. ( C ) Stills from confocal microscopy tracking GFP-HP1a in embryos laid from mothers either heterozygous or homozygous for grp. Note that grp embryos enter mitosis 13 before completing S phase, leading to anaphase bridges (17:00). Bar, 4 µm. The start of interphase 13 was set as 0:00. ( D ) Plot of the average number of HP1a foci per nucleus over time in control embryos (orange) or grp embryos (black). Each line represents data from a different embryo, and foci of GFP-HP1a were counted in ∼15 nuclei per embryo. ( E ) Inducing an early mitosis 14 by expression of twine (cdc25) interrupts heterochromatin formation. Embryos expressing GFP-HP1a were filmed following injection of twine mRNA during cycle 13. Bar, 3 µm. The beginning of cycle 14 was set as 0:00.

    Article Snippet: JabbaTrap and Cdc25 ( twine ) mRNAs were synthesized using the CellScript T7 mRNA production system and injected at a concentration of 600 ng/μL. dsRNA against the cyclins A, B, and B3 was prepared as described in .

    Techniques: Imaging, Injection, Labeling, Confocal Microscopy, Control, Expressing